Thermo Fisher Scientific GFAP Monoclonal Antibody (GA5), eFluor 615, eBioscience

Thermo Fisher Scientific GFAP Monoclonal Antibody (GA5), eFluor 615, eBioscience

Applications and Tested Dilutions

Product Specifications

Additional Formats:

• Unconjugated
• Alexa Fluor 488
• eFluor 570
• eFluor 660
• Request custom conjugation

Product Specific Information

This GA5 monoclonal antibody reacts with human, mouse, rat, chicken, rabbit, and pig glial fibrillary acidic protein (GFAP). GFAP is a 49-kDa type III intermediate filament protein expressed in neural tissues, distinguishing astrocytes from other glial cells during CNS development.
Three splice variants exist, with alpha-GFAP being predominant. GFAP co-assembles with vimentin and nestin, though not required for filament assembly. Mutations in GFAP cause Alexander disease and overexpression is observed in glial-derived tumors.

Applications Reported: Suitable for immunocytochemistry (ICC) and immunohistochemistry on frozen sections (IHC-F).
Applications Tested: Validated on fixed and permeabilized C6 cells ≤10 µg/mL. Not validated for flow cytometry.
Filter Recommendation: Use filters capturing 615 nm emission (e.g., Excitation 560/55, 585LP, Emission 645/75). Alexa Fluor® 594 filters are compatible.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

GFAP (Glial fibrillary acidic protein) is a class III intermediate filament protein expressed in astrocytes and astroglia of the CNS, satellite cells of peripheral ganglia, and non-myelinating Schwann cells. Also expressed in neural stem cells, lens epithelium, Kupffer cells, and certain tumors.
GFAP serves as a marker for astrocytic cells and is critical in distinguishing astrocytes during development. Mutations in GFAP lead to Alexander disease. Multiple isoforms arise via alternative splicing.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

제품 이미지