Thermo Fisher Scientific GFAP Monoclonal Antibody (GA5), eFluor 615, eBioscience
GFAP 단백질을 인식하는 GA5 클론의 마우스 단일클론 항체로, eFluor 615 형광 표지. 인간, 마우스, 랫 등 다양한 종 반응성. 신경 조직 내 성상세포 마커로 사용. 면역형광 및 면역조직화학 분석에 적합.
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Thermo Fisher Scientific GFAP Monoclonal Antibody (GA5), eFluor 615, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 3 publications |
| Immunohistochemistry (IHC) | - | View 19 publications |
| Immunohistochemistry (Frozen) (IHC-F) | Assay-Dependent | View 2 publications |
| Immunocytochemistry (ICC/IF) | 10 µg/mL | View 5 publications |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Chicken, Human, Mouse, Pig, Rabbit, Rat |
| Published Species | Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GA5 |
| Conjugate | eFluor™ 615 |
| Excitation / Emission Max | 595 / 614 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10804642 |
Additional Formats:
Product Specific Information
This GA5 monoclonal antibody reacts with human, mouse, rat, chicken, rabbit, and pig glial fibrillary acidic protein (GFAP). GFAP is a 49-kDa type III intermediate filament protein expressed in neural tissues, distinguishing astrocytes from other glial cells during CNS development.
Three splice variants exist, with alpha-GFAP being predominant. GFAP co-assembles with vimentin and nestin, though not required for filament assembly. Mutations in GFAP cause Alexander disease and overexpression is observed in glial-derived tumors.
Applications Reported: Suitable for immunocytochemistry (ICC) and immunohistochemistry on frozen sections (IHC-F).
Applications Tested: Validated on fixed and permeabilized C6 cells ≤10 µg/mL. Not validated for flow cytometry.
Filter Recommendation: Use filters capturing 615 nm emission (e.g., Excitation 560/55, 585LP, Emission 645/75). Alexa Fluor® 594 filters are compatible.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
GFAP (Glial fibrillary acidic protein) is a class III intermediate filament protein expressed in astrocytes and astroglia of the CNS, satellite cells of peripheral ganglia, and non-myelinating Schwann cells. Also expressed in neural stem cells, lens epithelium, Kupffer cells, and certain tumors.
GFAP serves as a marker for astrocytic cells and is critical in distinguishing astrocytes during development. Mutations in GFAP lead to Alexander disease. Multiple isoforms arise via alternative splicing.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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