Thermo Fisher Scientific alpha Actinin 4 Recombinant Rabbit Monoclonal Antibody (ARC1959)
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Thermo Fisher Scientific의 alpha Actinin 4 항체(ARC1959)는 인간, 마우스, 랫트 시료에 반응하는 재조합 토끼 단일클론 항체입니다. Western blot, ICC/IF, ELISA, IP 등 다양한 응용에 적합하며, 높은 특이성과 안정성을 제공합니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific alpha Actinin 4 Recombinant Rabbit Monoclonal Antibody (ARC1959)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5 µg–4 µg antibody for 200 µg–400 µg extracts |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC1959 |
| Immunogen | Synthetic peptide corresponding to amino acids 1–100 of human alpha-Actinin-4 (O43707) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.55 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2897979 |
Product Specific Information
- Positive Samples: HeLa, 293T, Jurkat, Mouse brain, Mouse spleen, Rat lung
- Immunogen sequence:
MVDYHAANQS YQYGPSSAGN GAGGGGSMGD YMAQEDDWDR DLLLDPAWEK QQRKTFTAWC NSHLRKAGTQ IENIDEDFRD GLKLMLLLEV ISGERLPKPE
Target Information
Alpha actinins belong to the spectrin gene superfamily, which includes diverse cytoskeletal proteins such as alpha and beta spectrins and dystrophins.
Alpha actinin is an actin-binding protein with multiple roles in different cell types:
- In nonmuscle cells, it is found along microfilament bundles and adherens-type junctions, binding actin to the membrane.
- In muscle cells, isoforms are localized to the Z-disc and dense bodies, anchoring myofibrillar actin filaments.
This gene encodes a nonmuscle alpha actinin isoform concentrated in the cytoplasm and is thought to be involved in metastatic processes.
Mutations in this gene have been associated with focal and segmental glomerulosclerosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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