Thermo Fisher Scientific V5 Tag Monoclonal Antibody (SV5-Pk1)
V5 태그 단백질 검출용 단클론 항체로 다양한 응용(WB, IHC, ELISA 등)에 사용 가능. SV5 바이러스 유래 항원 인식. 마우스 IgG2a 아이소타입, 단백질 A 정제. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 | View 1 publication |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | – |
| Immunocytochemistry (ICC/IF) | 1:100 | – |
| Flow Cytometry (Flow) | Assay-dependent | – |
| ELISA | 1:1,000–1:5,000 | – |
| Immunoprecipitation (IP) | Assay-dependent | View 1 publication |
| Radioimmune Assays (RIA) | Assay-dependent | – |
| Cytotoxicity Assay (CT) | – | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Published Species | Tag, Virus |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SV5-Pk1 |
| Immunogen | Paramyxovirus SV5, Simian-Virus 5 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1959285 |
Product Specific Information
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Epitope tags provide a method to localize gene products in various cell types, study protein topology and complexes, identify associated proteins, and characterize newly identified or low-abundance proteins when protein-specific antibodies are unavailable.
The V5 epitope tag is derived from a small epitope (Pk) on the P and V proteins of the paramyxovirus SV5.
The V5 tag is typically used with all 14 amino acids (GKPIPNPLLGLDST) but can also be used with a shorter 9 amino acid sequence (IPNPLLGLD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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