Thermo Fisher Scientific CD45 Monoclonal Antibody (2D1), NovaFluor Yellow 590, eBioscience

Thermo Fisher Scientific CD45 Monoclonal Antibody (2D1), NovaFluor Yellow 590

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.8 µg)/test

Product Specifications

Additional Available Formats

• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant Violet 711
• eFluor 450
• FITC
• NovaFluor Blue / Red / Violet / Yellow series (multiple wavelengths)
• PE, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 780
• Request custom conjugation available

Product Specific Information

The 2D1 monoclonal antibody reacts with all isoforms of human CD45 (Leukocyte Common Antigen, LCA), expressed by all hematopoietic cells except erythrocytes and platelets. CD45 possesses tyrosine phosphatase activity and plays a key role in lymphocyte activation.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and validated for flow cytometry on normal human peripheral blood cells. Recommended usage: 5 µL (0.8 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining. Add 5 µL per sample before combining with cells.
• For single-color controls, use 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology, engineered for optimized fluorescent signatures minimizing spillover and spread.

Excitation: 552 nm
Emission: 592 nm
Laser: 561 nm (Yellow)

Target Information

CD45 (LCA) is a receptor-type protein tyrosine phosphatase expressed on all nucleated hematopoietic cells, absent on non-hematopoietic tissues. It is critical for lymphocyte development and antigen signaling, regulating Src-family kinases and JAK kinases. CD45 isoforms differ by extracellular domain splicing and play roles in membrane localization, apoptosis, and immune regulation.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

제품 이미지