Thermo Fisher Scientific CD45 Monoclonal Antibody (2D1), NovaFluor Yellow 610, eBioscience

Thermo Fisher Scientific CD45 Monoclonal Antibody (2D1), NovaFluor Yellow 610, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.8 µg)/test

Product Specifications

Additional Formats Available

• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant Violet 711
• eFluor 450
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 585
• NovaFluor Blue 610-30S
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 660-40S
• NovaFluor Blue 690
• NovaFluor Blue 725
• NovaFluor Blue 760
• NovaFluor Blue 800
• NovaFluor Red 660
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor UV 765
• NovaFluor Violet 690
• NovaFluor Violet 800
• NovaFluor Yellow 590
• NovaFluor Yellow 660
• NovaFluor Yellow 700
• NovaFluor Yellow 730
• NovaFluor Yellow 755
• NovaFluor Yellow 810
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 780
• Request custom conjugation available

Product Specific Information

The 2D1 monoclonal antibody reacts with all isoforms of human CD45 (Leukocyte Common Antigen, LCA). CD45 is expressed by all hematopoietic cells except circulating erythrocytes and platelets. Its cytoplasmic domain exhibits tyrosine phosphatase activity, playing a key role in lymphocyte activation.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and validated for flow cytometry of normal human peripheral blood cells. Recommended use: 5 µL (0.8 µg) per test (100 µL total volume, 10⁵–10⁸ cells/test).

Important Notes:

• NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining and should be added before combining antibodies with cells (5 µL/sample).
• For single-color controls: use 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology based on nucleic acid dye structures engineered for minimal spillover and spread impact.

Excitation: 552 nm
Emission: 612 nm
Laser: 561 nm (Yellow)

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed on all nucleated hematopoietic cells. It comprises ~10% of lymphocyte surface proteins and is absent from non-hematopoietic tissues. CD45 regulates Src-family kinases, T/B-cell receptor signaling, cytokine receptor pathways, and integrin-mediated survival signaling. Multiple isoforms (e.g., CD45RA) arise via alternative splicing, differing in extracellular domains but sharing identical transmembrane and cytoplasmic regions.

For Research Use Only. Not for use in diagnostic procedures.

제품 이미지