Thermo Fisher Scientific IFN gamma Polyclonal Antibody, PeproTech
Rabbit 유래의 Human IFN-gamma 특이적 폴리클로날 항체로, Western blot, ELISA 등 다양한 응용에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 동결건조 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
- 카탈로그번호
- 500-P32-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IFN gamma Polyclonal Antibody, PeproTech
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | – |
| Immunohistochemistry (IHC) | – | 3 publications |
| Immunocytochemistry (ICC/IF) | – | 1 publication |
| Flow Cytometry (Flow) | – | 1 publication |
| ELISA | 0.5–2.0 µg/mL | 1 publication |
| Neutralization (Neu) | – | 3 publications |
| In vitro Assay (IV) | – | 5 publications |
| Immunostaining (IS) | – | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Bacteria, Hamster, Human, Protozoa |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human IFN-gamma |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930395 |
Product Specific Information
Amino Acid Sequence of Recombinant Protein:
MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IFN-gamma. Anti-Human IFN-gamma-specific antibody was purified by affinity chromatography employing an immobilized Human IFN-gamma matrix.
Sandwich ELISA:
To detect Human IFN-gamma by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IFN-gamma (500-P32Bt) as a detection antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human IFN-gamma.
Western Blot:
To detect Human IFN-gamma by Western blot, use at 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human IFN-gamma is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Target Information
IFN gamma (Interferon gamma, Type II interferon)
- A macrophage activation factor and immune interferon produced primarily by T-lymphocytes and NK cells in response to antigens and cytokines.
- Dimeric glycoprotein (~45 kDa) composed of two 146 amino acid subunits.
- Appears as 25, 20, and 15.5 kDa bands on SDS-PAGE due to differential glycosylation.
- Species specific; human IFN gamma does not cross-react with mouse.
- Functions include antiviral and antiproliferative activity, induction of MHC I/II, macrophage activation, and enhanced immunoglobulin secretion.
- Acts via IFN gamma receptor I/II and JAK-STAT pathway.
- Expression is upregulated by IL-2, FGF basic, EGF, and downregulated by vitamin D3 or DMN.
- Mutations in IFN gamma gene are associated with aplastic anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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