Thermo Fisher Scientific PD-L1 Fc Polyclonal Antibody, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:

FTVTVPKDLY VVEYGSNMTI ECKFPVEKQL DLAALIVYWE MEDKNIIQFV HGEEDLKVQH SSYRQRARLL KDQLSLGNAA LQITDVKLQD AGVYRCMISY GGADYKRITV KVNAPYNKIN QRILVVDPVT SEHELTCQAE GYPKAEVIWT SSDHQVLSGK TTTTNSKREE KLFNVTSTLR INTTTNEIFY CTFRRLDPEE NHTAELVIPE LPLAHPPNER GGPKSCDKTH TCPPCPAPEL LGGPSVFLFP PKPKDTLMIS RTPEVTCVVV DVSHEDPEVK FNWYVDGVEV HNAKTKPREE QYNSTYRVVS VLTVLHQDWL NGKEYKCKVS NKALPAPIEK TISKAKGQPR EPQVYTLPPS RDELTKNQVS LTCLVKGFYP SDIAVEWESN GQPENNYKTT PPVLDSDGSF FLYSKLTVDK SRWQQGNVFS CSVMHEALHN HYTQKSLSLS PGK.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human PD-L1 Fc. Anti-Human PD-L1 Fc-specific antibody was purified by affinity chromatography employing an immobilized Human PD-L1 Fc matrix.

Sandwich ELISA:
To detect Human PD-L1 Fc by sandwich ELISA (using 100 µL/well), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human PD-L1 Fc (500-P321BT) as a detection antibody, allows the detection of at least 2000–4000 pg/mL of Recombinant Human PD-L1 Fc.

Western Blot:
To detect Human PD-L1 Fc by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human PD-L1 Fc is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a B7 family member that binds to the programmed death receptor 1 (PD-1), which negatively regulates T-cell receptor (TCR) signaling. Upon ligation, PD-L1 decreases TCR-mediated proliferation and cytokine production. PD-L1 expression is abundant in many murine and human cancers and can be upregulated by IFN-gamma stimulation, suggesting its role in tumor immune evasion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.