Thermo Fisher Scientific TID1 Polyclonal Antibody
Human TID1 단백질을 인식하는 Rabbit Polyclonal 항체로, IHC(P) 및 ICC/IF에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, PBS 완충액(40% 글리세롤 포함) 중 액상 형태로 제공. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human DNAJA3. Recombinant protein control fragment (Product #RP-98662). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2790675 |
Product Specific Information
Immunogen sequence:
DPEELFRKIF GEFSSSSFGD FQTVFDQPQE YFMELTFNQA AKGVNKEFTV NIMDTCERCN GKGNEPGTKV QHCHYCGGSG METINTGPFV MRST
Target Information
TID1 is a human homolog of the Drosophila tumor suppressor lethal (2) tumorous imaginal discs, l(2)tid, and encodes two mitochondrial matrix localized splice variants, hTid-1S and hTid-1L. These proteins belong to the DnaJ family, acting as cochaperones for mitochondrial Hsp70. They contain a conserved J domain that binds Hsp70 chaperones and activates their ATPase activity.
Expression of hTid-1L increases apoptosis induced by DNA damaging agents such as mitomycin-C and TNF-α, while a J-domain mutant can suppress apoptosis. Conversely, the J-domain mutant of hTid-1S increases apoptosis. Expression of these variants affects cytochrome c release and caspase 3 activation but not caspase 8. The relative abundance of these splice variants may regulate mitochondrial apoptotic signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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