Thermo Fisher Scientific CD45 Monoclonal Antibody (30-F11), Super Bright 600, eBioscience
Mouse CD45 (30-F11) 단클론 항체로 Super Bright 600 형광체 결합. Flow cytometry에 최적화되어 있으며 모든 CD45 isoform을 인식. 높은 형광 안정성과 명확한 세포 표지 제공. 연구용으로만 사용.
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Thermo Fisher Scientific CD45 Monoclonal Antibody (30-F11), Super Bright 600, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 38 publications |
| Immunohistochemistry (PFA fixed) | - | View 1 publication |
| Immunohistochemistry (Frozen) | - | View 3 publications |
| Immunocytochemistry (ICC/IF) | - | View 9 publications |
| Flow Cytometry (Flow) | 0.25 µg/test | View 207 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), Super Bright™ 600, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 30-F11 |
| Conjugate | Super Bright™ 600 |
| Excitation / Emission Max | 414 / 601 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2637148 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488, 532, 561, 660, 700
- APC, APC-eFluor 780, Biotin
- Brilliant UV series (395–805)
- Brilliant Violet series (421–786)
- eFluor 450, 506
- FITC, Functional Grade
- NovaFluor Blue, Red, Violet, Yellow series
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436, 645, 702, 780
- Request custom conjugation available
Product Specific Information
The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45 (Leukocyte Common Antigen, LCA). CD45 is expressed on all hematopoietic cells except mature erythrocytes and platelets. The cytoplasmic domain has tyrosine phosphatase activity essential for lymphocyte activation.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Mouse bone marrow cells, ≤0.25 µg/test (100 µL volume, 10⁵–10⁸ cells/test). Optimize titration for best results.
- Fluorochrome Information: Super Bright 600 is excited at 405 nm and emits at 600 nm. Recommended filter: 610/20 bandpass.
- Staining Buffer: Use Super Bright Complete Staining Buffer (Product # SB-4401) when combining multiple Super Bright dyes to reduce non-specific interactions.
- Light Sensitivity: Protect from light to prevent photo-induced oxidation.
- Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 2–8°C in the dark with minimal impact on brightness.
- Laser Compatibility: Violet laser (405 nm) excitation; emission 600 nm.
- Note: Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed on all nucleated hematopoietic cells (~10% of lymphocyte surface proteins). It regulates Src-family kinases and antigen receptor signaling, crucial for T- and B-cell activation and cytokine receptor regulation. CD45 isoforms arise via alternative splicing and differ in extracellular domains. It also modulates integrin signaling, apoptosis, and various immune functions.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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