Thermo Fisher Scientific IL-2 Monoclonal Antibody (MQ1-17H12), Brilliant Ultra Violet 737, eBioscience

Thermo Fisher Scientific IL-2 Monoclonal Antibody (MQ1-17H12), Brilliant Ultra Violet 737, eBioscience

Applications

• Flow Cytometry (Intracellular staining)

Tested Dilution: 5 µL (0.25 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 532
• APC
• Brilliant UV 395
• Brilliant UV 615
• Brilliant Violet 421
• Brilliant Violet 480
• eFluor 450
• FITC
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The MQ1-17H12 antibody reacts with human interleukin-2 (IL-2), a 17 kDa T cell growth factor and major immunoregulatory cytokine. It is a non-neutralizing antibody suitable for intracellular staining and flow cytometric analysis.

This antibody has been pre-diluted and tested using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00). Recommended usage: 5 µL (0.25 µg) per test, defined for a 100 µL final staining volume. Suitable for 10⁵–10⁸ cells/test.

Brilliant Ultra Violet™ 737 (BUV737) emits at 732 nm and is intended for cytometers equipped with a 355 nm UV laser. Ensure instrument compatibility before use.

When using multiple polymer dye-conjugated antibodies (e.g., Super Bright, Brilliant Violet, Brilliant Ultra Violet), use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to reduce non-specific interactions.

Light Sensitivity: Protect vial and stained samples from light.
Fixation: Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54).
Methanol Compatibility: Compatible with short-term methanol fixation (<1 hour).
Excitation: 355 nm
Emission: 732 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

Interleukin 2 (IL-2) is an immunomodulatory cytokine essential for proliferation of activated T cells and differentiation of B cells, NK cells, monocytes, and macrophages. IL-2 signals through IL-2R, a heterotrimeric receptor complex sharing its gamma chain with IL-4 and IL-7. Monoallelic IL-2 expression in thymocytes represents a unique regulatory mechanism. Gene disruption studies in mice highlight IL-2’s critical role in immune homeostasis.

For Research Use Only. Not for diagnostic use or resale without authorization.

제품 이미지