Thermo Fisher Scientific CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH5), Brilliant Ultra Violet 737, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.25 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 660
• APC
• Biotin
• Brilliant UV 615
• Brilliant UV 661
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 650
• Brilliant Violet 786
• Functional Grade
• NovaFluor Red 700
• NovaFluor Red 755
• NovaFluor Yellow 690
• NovaFluor Yellow 700
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 780
• Request custom conjugation

Product Specific Information

The MIH5 monoclonal antibody reacts with mouse B7-H1 (PD-L1), a member of the B7 family with a molecular weight of approximately 40 kDa. B7-H1 is expressed on multiple leukocyte types including T, B, NK, and dendritic cells, and functions as a ligand for PD-1. Interaction of PD-1 with PD-L1 or PD-L2 inhibits T and B cell responses. MIH5 is a blocking antibody.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes; ≤0.25 µg/test recommended.
Each test corresponds to staining a cell sample in 100 µL volume (10⁵–10⁸ cells/test). Optimize dilution empirically.

Fluorochrome Information:
Brilliant Ultra Violet™ 737 (BUV737) emits at 732 nm and is used with UV (355 nm) lasers. Ensure cytometer compatibility.

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401) or Brilliant Stain Buffer (00-4409) to minimize non-specific polymer interactions.

Light Sensitivity:
Protect from light to prevent photo-induced oxidation.

Fixation:
Samples can be stored in IC Fixation Buffer (00-8222) or 1-step Fix/Lyse Solution (00-5333) for up to 3 days at 4°C in the dark.
BUV737 is compatible with short-term methanol fixation but avoid storage in methanol-containing buffers beyond one hour.

Excitation / Emission: 355 nm / 732 nm
Laser: Ultraviolet laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

Programmed death receptor ligand 1 (PD-L1, B7-H1) is a B7 family member that binds PD-1 receptor, negatively regulating T-cell receptor signaling. PD-L1 reduces TCR-mediated proliferation and cytokine production and is abundantly expressed in murine and human cancers, upregulated by IFN-γ. PD-L1 is implicated in tumor immune evasion.

For Research Use Only. Not for use in diagnostic procedures.

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