Thermo Fisher Scientific Integrin beta 7 Recombinant Rat Monoclonal Antibody (FIB27)

Applications

Flow Cytometry (Flow)

• Tested Dilution: Assay-dependent

Immunoprecipitation (IP)

• Tested Dilution: Assay-dependent

Inhibition Assays (IA)

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

Specificity:
FIB27 binds specifically to both human and mouse integrin beta-7 at epitope region Dl, which is involved in all α4β7-mediated adhesion events.
Does not compete with DAKT32 for binding.
FIB27 blocks LS722-induced TK1 cell aggregation (mAb LS722 is an activating anti-α4β7 antibody and induces aggregation via an α4β7-dependent pathway).

Target Information

Integrin beta 7 associates with integrin alpha 4 (CD49d) to form the alpha 4 beta 7 integrin (LPAM-1), expressed on intraepithelial lymphocytes.
It also associates with alpha E (CD103) to form the alpha E beta 7 integrin (HML-1), expressed on T cells adjacent to mucosal epithelium and intraepithelial lymphocytes.
Ligands for LPAM-1 include VCAM-1 (CD106), MAdCAM-1, and fibronectin, while the ligand of HML-1 is E-cadherin (CD324).
Integrin beta 7 plays an important role in the adhesion of leukocytes to endothelial cells, promoting the transmigration of leukocytes to extravascular spaces during the inflammatory response.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.