Thermo Fisher Scientific BRCC3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1 µg/mL

View 2 publications 2 publications

Immunohistochemistry (IHC)

2.5 µg/mL

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

-

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

20 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

An 18 amino acid peptide from near the amino terminus of human BRCC36. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:BRCC3,uniProtId:P46736-1,ncbiNodeId:9606,antigenRange:1,antigenLength:316,antigenImageFileName:PA5-20426_BRCC3_P46736-1_Rabbit.svg,antigenImageFileNamePDP:PA5-20426_BRCC3_P46736-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11154606

Product Specific Information

A suggested positive control is MCF7 cell lysate.

PA5-20426 can be used with blocking peptide PEP-0543.

Target Information

Together with the breast and ovarian predisposition proteins BRCA1 and BRCA2 and RAD51 and BRCC45, BRCC36 forms a holoenzyme complex that possesses a ubiquitin E3 ligase activity. Aberrant levels of BRCC36 were detected in sporadic breast tumors and depletion of either BRCC36 or BRCC45 by siRNA resulted in increased sensitivity to ionizing radiation and defects in the G2/M checkpoint, indicating that BRCC36 acts to enhance cellular survival following DNA damage. Recent experiments have shown that BRCC36 is essential for ionizing radiation-induced BRCA1 phosphorylation and nuclear foci formation, suggesting that BRCC36 may be an important target in the treatment of radiation-resistant breast tumors. At least two isoforms of BRCC36 are known to exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.