Thermo Fisher Scientific CD197 (CCR7) Monoclonal Antibody (3D12), NovaFluor Yellow 700, eBioscience

Thermo Fisher Scientific CD197 (CCR7) Monoclonal Antibody (3D12), NovaFluor Yellow 700, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.6 µg)/test

Product Specifications

Additional Formats Available

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 563 / 661 / 737
• Brilliant Violet 421 / 480 / 711 / 786
• FITC
• NovaFluor Red 685 / 700 / 755
• PE / PE-Cyanine5.5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 600 / 645 / 780
• Request custom conjugation available

Product Specific Information

The 3D12 monoclonal antibody reacts with human CCR7 (CD197), also known as EBI-1. CCR7 is a G-protein-coupled chemokine receptor with seven membrane-spanning domains, functioning as a receptor for 6Ckine/SLC (CCL19 and CCL21).
CCR7 is expressed on T cells, distinguishing naive, central, and effector memory populations, and plays a role in migration of memory T cells to inflamed tissues. Expression is also found on dendritic cells (DCs), increasing during maturation and contributing to chemotaxis, endocytosis rate, survival, and maturation.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and tested by flow cytometry on human peripheral blood cells.
Recommended use: 5 µL (0.6 µg) per test (final volume 100 µL). Incubate ≥45 minutes at 2–8°C for optimal staining.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer (Cat. No. C001T06F01)

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.
• CellBlox Plus Blocking Buffer should be added to antibody cocktails before combining with cells (5 µL per sample).
• For single-color controls: 5 µL buffer per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology with engineered fluorescent signatures minimizing spillover and spread.

Excitation: 552 nm
Emission: 700 nm
Laser: 561 nm (Yellow)

Target Information

CCR7 is a member of the G protein-coupled receptor family involved in chemokine signaling. Induced by Epstein Barr virus (EBV), CCR7 mediates EBV effects on B lymphocytes and is expressed in blood, bone marrow, lymph node, and intestine.
It regulates migration of memory T cells and dendritic cell maturation. CCL19/ECL is a specific ligand. Related chemokine receptors include CCR1–CCR5 and Duffy antigen, all important for T cell chemotaxis and phagocytic cell migration.

For Research Use Only. Not for use in diagnostic procedures.

제품 이미지