Thermo Fisher Scientific Ly-6C Monoclonal Antibody (HK1.4), Functional Grade, eBioscience

Thermo Fisher Scientific Ly-6C Monoclonal Antibody (HK1.4), Functional Grade, eBioscience

Applications and Tested Dilution

Product Specifications

Available Conjugated Formats

• Alexa Fluor 488
• APC
• APC-eFluor 780
• eFluor 450
• PE
• PE-Cyanine7
• PerCP-Cyanine5.5
• Super Bright 436
• Request custom conjugation

Product Specific Information

The monoclonal antibody HK1.4 recognizes mouse Ly-6C, a GPI-linked protein of the Ly6 family. Ly-6C is expressed on monocytes/macrophages, endothelial cells, granulocytes, and subsets of lymphocytes. Expression varies among mouse strains:

• Ly6c.1 (C57Bl/6, SJL): stains both CD4 and CD8 cells
• Ly6c.2 (BALB/c, 3H/He): stains CD8 but not CD4 cells

In vitro, HK1.4 antibody can enhance proliferation and stimulate cytokine release.

Applications Reported: Flow cytometric analysis and functional assays.
Applications Tested: Flow cytometric analysis of mouse splenocytes, ≤0.25 µg/test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test. Antibody should be titrated for optimal performance.

Storage and Handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: >90% (SDS-PAGE).
Endotoxin Level: <0.001 ng/µg antibody (LAL assay).
Aggregation: <10% (HPLC).

Target Information

Granulocytes are white blood cells containing cytoplasmic granules, also known as polymorphonuclear leukocytes (PMN/PML). Their nuclei are typically lobed into three segments. Neutrophil granulocytes are the most abundant type. Granulocytes are released from bone marrow under regulation by complement proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.