
Thermo Fisher Scientific Goat anti-Chicken IgY (H+L) Secondary Antibody, Alexa Fluor 488
Alexa Fluor 488 형광을 결합한 Goat anti-Chicken IgY (H+L) 2차 항체로, IHC, ICC, Flow Cytometry 등에 사용 가능. 높은 형광 안정성과 감도를 제공하며, 488 nm 레이저에 최적화. 연구용으로만 사용.
- 카탈로그번호
- A11039
- 판매단위
- pk
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Thermo Fisher Scientific Goat anti-Chicken IgY (H+L) Secondary Antibody, Alexa Fluor 488
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (IHC) | 1–10 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–10 µg/mL |
| Flow Cytometry (Flow) | 1–10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chicken |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.5 |
| Contains | 5 mM sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient |
| RRID | AB_2534096 |
| Target | IgY |
| Antibody Form | Whole Antibody |
Additional Conjugate Formats
- Alexa Fluor 546
- Alexa Fluor 555
- Alexa Fluor 568
- Alexa Fluor 594
- Alexa Fluor 647
- AP
- Biotin
- FITC
- HRP
- Texas Red
- TRITC
- Request custom conjugation available
Product Specific Information
Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 488 dye is a bright, green-fluorescent dye ideally suited for the 488 nm laser line. It provides stable signal generation in imaging and flow cytometry, being pH-insensitive over a wide range.
High fluorescence quantum yield and photostability enable detection of low-abundance biological structures with high sensitivity. The dye can be attached to proteins at high molar ratios without significant self-quenching, resulting in brighter conjugates and more sensitive detection.
Typical labeling degree: 2–8 fluorophore molecules per IgG molecule (exact value indicated on certificate of analysis).
Usage Notes:
- Centrifuge conjugate solution briefly before use and use only the supernatant to remove aggregates.
- Recommended final concentration for most applications: 1–10 µg/mL.
- Product ships at room temperature.
Target Information
Anti-Chicken secondary antibodies are affinity-purified with well-characterized specificity for chicken immunoglobulins. They are useful for detection, sorting, or purification of target proteins.
Secondary antibodies increase versatility and sensitivity via signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically generated by immunizing host animals with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation) for high specificity.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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