Thermo Fisher Scientific Phospho-ELK1 (Thr417) Polyclonal Antibody
Human, Mouse, Rat에서 반응하는 Rabbit Polyclonal Antibody로, Thr417에서 인산화된 ELK1 단백질을 검출. Western blot, IHC(P), IP에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 고순도(>95%) 유지. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunoprecipitation (IP) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide derived from human Elk-1 around the phosphorylation site of Threonine 417 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2553630 |
Product Specific Information
- Detects endogenous ELK1 protein at ~45 kDa.
- Purity: >95% by SDS-PAGE.
Target Information
ELK1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors. It is phosphorylated by MAP kinase pathways at multiple serine/threonine motifs at its C-terminus. Phosphorylation, particularly at Ser383, is critical for transcriptional activation by ELK1. ELK1 acts as a direct target of activated MAP kinase and serves as a nuclear target for the ras-raf-MAPK signaling cascade. Alternatively spliced transcript variants encoding the same protein have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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