Thermo Fisher Scientific TIN2 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:1,000

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:100–1:500

Product Specifications

Product Specific Information

Antibody detects endogenous levels of total TINF2.

Target Information

TRF1 is a negative regulator of telomere length maintenance. Two proteins, tankyrase and Tin2, which bind to mammalian TRF1, have been identified.
Tankyrase localizes to telomeres by binding to TRF1 through its ankyrin repeats and exhibits PARP activity functioning as acceptors for ADP-ribosylation.
ADP-ribosylation of TRF1 diminishes its ability to bind to telomeric DNA, suggesting telomere function in human cells is regulated by poly (ADP)-ribosylation.
TIN2 was isolated as an interacting partner of TRF1 from a yeast two-hybrid cDNA library screening. Tin2 localizes to telomeres and is essential for proper regulation of telomere length.
TIN2, like TRF1, is widely and constitutively expressed, suggesting cooperative action to counterbalance telomere elongation by telomerase.
However, endogenous TIN2 is not highly expressed and may be difficult to visualize by immunostaining in certain cell systems.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.