Thermo Fisher Scientific Calnexin Monoclonal Antibody (CANX, 1543)
Calnexin 단백질을 인식하는 Mouse monoclonal 항체로, WB 및 IHC에 최적화되어 있습니다. Human 시료에 반응하며, Protein A/G로 정제된 액상 형태입니다. ER 내 단백질 폴딩 연구 및 Calnexin 관련 단백질 분석에 적합합니다.
- 카탈로그번호
- 821-MSM3-P1ABX
- 판매단위
- pk
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CANX, 1543 |
| Immunogen | Recombinant N-terminal fragment of human Calnexin protein (around aa 1–300) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Antibody is tested in direct ELISA (use at 2–4 µg/mL for coating).
- For Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 min.
Target Information
Calnexin (also known as IP90, p88, and p90) is an approximately 90 kDa integral membrane protein of the endoplasmic reticulum (ER). It functions as a molecular chaperone involved in proper folding and assembly of polypeptides into multisubunit proteins.
Calnexin associates with MHC class I heavy chains, partial complexes of the T cell receptor, and B cell membrane immunoglobulin, but not with completed receptor complexes. It retains incompletely or improperly folded proteins within the ER.
Soluble ER proteins often contain the KDEL sequence for ER retention, whereas calnexin, as an integral membrane protein, lacks KDEL but has positively charged cytosolic residues ensuring ER localization.
Calnexin structure includes:
- ER luminal domain: 461 amino acids
- Transmembrane segment: 22 amino acids
- Cytoplasmic tail: 89 amino acids
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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