Thermo Fisher Scientific AKAP8 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 480-692 of human AKAP8 (NP_005849.1). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:AKAP8,uniProtId:O43823-1,ncbiNodeId:9606,antigenRange:480-692,antigenLength:692,antigenImageFileName:PA5-120850_AKAP8_O43823-1_Rabbit.svg,antigenImageFileNamePDP:PA5-120850_AKAP8_O43823-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.08 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2914422

Product Specific Information

Positive test controls include: LO2, 293T, BxPC-3, HeLa, Jurkat, mouse thymus. The target is usually found in the following locations: Nucleus matrix.

Immunogen sequence: HCLACDMLIP AQPQLLQRHL HSVDHNHNRR LAAEQFKKTS LHVAKSVLNN RHIVKMLEKY LKGEDPFTSE TVDPEMEGDD NLGGEDKKET PEEVAADVLA EVITAAVRAV DGEGAPAPES SGEPAEDEGP TDTAEAGSDP QAEQLLEEQV PCGTAHEKGV PKARSEAAEA GNGAETMAAE AESAQTRVAP APAAADAEVE QTDAESKDAV PTE

Target Information

The type II cAMP-protein kinase (PKA) is a multifunctional kinase with a broad range of substrates. Specificity of PKA signaling is thought to be mediated by the compartmentalization of the kinase to specific sites within the cell. To maintain this specific localization, the R subunit (RII) of PKA interacts with specific RII-anchoring proteins. The family of RII-anchoring proteins has been designated A-kinase anchoring proteins (AKAP). AKAP 95, also known as AKAP 8, is a nuclear matrix protein predominantly expressed in liver, heart, pancreas, kidney and skeletal muscle. During mitosis, AKAP 95 is recruited to the chromosomes and plays an essential role in mitotic progression. Characteristic of its family, AKAP 95 participates in PKA signaling through an interaction with the RII regulatory subunit. In addition, AKAP 95 forms a complex with HA95 and HDAC3 and is required for the deacetylation of Histone H3 in mitosis.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.