Invitrogen™

pBudCE4.1 Mammalian Expression Vector

The pBudCE4.1 vector is designed for the independent expression of two genes from a single plasmid in mammalian cells. Using자세히 알아보기

The pBudCE4.1 vector is designed for the independent expression of two genes from a single plasmid in mammalian cells. Using pBudCE4.1 to generate stable mammalian expression cell lines ensures that there is an equivalent copy number of each gene in the cell. This can eliminate variable expression due to differences in gene copy number. pBudCE4.1 provides expression cassettes withthe following features:

The CMV promoter for high-level transcription of genes with an optional c-myc epitope tag for rapid detection and 6xHis sequence for simple purification

• The human EF-1α promoter for high-level expression of genes with an optional V5 epitope tag for rapid detection and 6xHis sequence for simple purification
• The Sh ble (ZeoR) gene for efficient selection in both mammalian cells and _E. coli_with the selection agent Zeocin™

사양

구성 또는 유도성 시스템Constitutive

배달 유형Transfection

프로모터EF-1α, CMV

제품 유형Mammalian Expression Vector

선택 제제(진핵)Zeocin™

단백질 태그His Tag (6x), V5 Epitope Tag, c-Myc Epitope Tag

클로닝 방법Restriction Enzyme/MCS

수량20 μg

벡터pBud

용도(애플리케이션)Multi-Gene Expression

Unit Size20 µg