Invitrogen™

pLenti7.3/V5-DEST™ Gateway™ Vector Kit

The pLenti7.3/V5-DEST™ Gateway™ Vector Kit contains the Gateway™-adapted ViraPower™ HiPerform™ lentiviral expression vector, pLenti7.3/V5-DEST™ for easy recombination-based cloning and high-level자세히 알아보기

The pLenti7.3/V5-DEST™ Gateway™ Vector Kit contains the Gateway™-adapted ViraPower™ HiPerform™ lentiviral expression vector, pLenti7.3/V5-DEST™ for easy recombination-based cloning and high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti7.3/V5-DEST™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements. In addition, the vector allows for an accurate determination of titer of functional lentivirus in just two days using Emerald Green Fluorescent Protein (EmGFP).

Advantages

• Transient expression
• Short-term experiments
• High-throughput screening
• 2 day titer of functional virus using EmGFP
• Flexible and versatile Gateway™ recombination cloning technology

Key Features

• WPRE (Woodchuck Posttranscriptional Regulatory Element) from the woodchuck hepatitis virus, allows increased transgene expression and cPPT (Polypurine Tract) from the HIV-1 integrase gene, increases the copy number of lentivirus integrating into the host genome
• Human cytomegalovirus (CMV) immediate early promoter to control high-level expression of the gene of interest
• SV40 promoter driving expression of EmGFP
• Emerald Green Fluorescent Protein (EmGFP) allows easy determination of Lentiviral titers by flow cytometry and monitor transduced cells.

Kit Includes

• pLenti7.3/V5-DEST™ Gateway™ Vector box
• One Shot™ Stbl3™ chemically competent E. coli (Cat. No. C7373-03)

Related SKUs

• 293FT Cell Line (Cat. No. R70007)
• ViraPower™ Lentiviral Support Kit (K4970-00)
• ViraPower™ Lentiviral Packaging Mix (K4975-00)
• Lipofectamine™ 2000 (11668-019, 11668-027)

사양

구성 또는 유도성 시스템Constitutive

배달 유형Lentiviral

프로모터CMV

제품 유형Lentiviral Expression Vector

리포터 유전자GFP (EmGFP)

선택 제제(진핵)None

단백질 태그V5 Epitope Tag

클로닝 방법Gateway

수량1 Kit

벡터pLenti

제품라인ViraPower™, Gateway™, HIPERFORM™

용도(애플리케이션)Reporter Assays, Viral Expression

Unit Size1 kit