Invitrogen™

pFRT/_lac_Zeo Vector

pFRT/lacZeo and pFRT/lacZeo2 were constructed for establishing Flp-In™ Cell Lines that contain a single FRT site. Each vector includes a:•자세히 알아보기

pFRT/_lac_Zeo and pFRT/_lac_Zeo2 were constructed for establishing Flp-In™ Cell Lines that contain a single FRT site. Each vector includes a:

• FRT site for subsequent targeted integration of a Flp-In™ expression vector
Z-Zeocin™ fusion for selection of stable integrants with Zeocin™

These vectors differ in the SV40 promoter that drives expression of the _lac_Z-Zeocin™ fusion. pFRT/_lac_Zeo2 contains a truncated version of the SV40 promoter. Use of this vector facilitates the isolation of clones that have integrated the vector near enhancer elements in the genome. Selection of stable clones using pFRT/_lac_Zeo2 may allow easier isolation of clones that express a gene of interest at higher levels.

사양

구성 또는 유도성 시스템Constitutive

배달 유형Transfection

프로모터SV40

제품 유형Flp-In System Expression Vector

리포터 유전자Beta-Gal (lacZ)

선택 제제(진핵)Zeocin™

단백질 태그Untagged

수량20 μg

벡터pFRT

제품라인Flp-In™

용도(애플리케이션)Targeted Integration

Unit Size20 µg