Thermo Fisher Scientific UFD1L Monoclonal Antibody (OTI4C7)
UFD1L 단백질을 인식하는 Thermo Fisher Scientific의 마우스 단클론 항체. Western blot 및 파라핀 조직 IHC에 적합. 고순도 친화 크로마토그래피 정제, PBS/BSA/glycerol 완충액에 보관. -20°C에서 보관하며 동결/해동 반복은 피해야 함.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4C7 |
| Immunogen | Full length human recombinant protein of UFD1L produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2725624 |
Target Information
Ubiquitin-mediated proteolysis involves transferring ubiquitin (Ub) to lysine residues on target proteins, leading to their degradation by the 26S proteasome. Ub-fusions are processed by Ub-specific proteases (UBps) or by the Ub-fusion degradation (UFD) pathway. The UFD1 protein, first identified in S. cerevisiae and later in humans as UFD1L, plays a role in regulating Ub-fusion degradation. In vitro, UFD1 attenuates degradation of Ub-fusions with substitutions at the Gly76 residue by promoting selective multiubiquitination. Mutations in the UFD1 gene are linked to CATCH22 syndrome, characterized by cardiac defects, cleft palate, and hypocalcemia, indicating the pathway’s importance in developmental processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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