Thermo Fisher Scientific Troponin I Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 0.2–1 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 4–8 µg/mL

Product Specifications

Product Specific Information

Peptide sequence:
MPEVERKPKI TASRKLLLKS LMLAKAKECW EQEHEEREAE KVRYLAERIP

Sequence homology:
Cow: 93%; Dog: 93%; Goat: 79%; Guinea Pig: 93%; Horse: 93%; Human: 100%; Mouse: 100%; Rabbit: 93%; Rat: 100%

Target Information

Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles.
Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of three subunits that form the troponin complex of the thin filaments of striated muscle.
TnI is the inhibitory subunit, blocking actin-myosin interactions and mediating muscle relaxation.

The TnI subfamily contains three genes:

• TnI-skeletal-fast-twitch
• TnI-skeletal-slow-twitch
• TnI-cardiac

TnI-fast and TnI-slow are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while TnI-cardiac is expressed exclusively in cardiac muscle tissue.
This gene encodes the Troponin-I-skeletal-slow-twitch protein, expressed in cardiac and skeletal muscle during early development but restricted to slow-twitch skeletal muscle fibers in adults.
The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.