Thermo Fisher Scientific DNMT1 Monoclonal Antibody (60B1220.1)
DNMT1 단백질을 표적하는 Mouse Monoclonal Antibody로 다양한 종에 반응하며, WB, IHC, ICC, Flow, IP, ChIP 등 여러 응용 가능. 고순도 Protein G 정제, 1 mg/mL 농도, 장기 보관 시 -20°C 권장.
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Thermo Fisher Scientific DNMT1 Monoclonal Antibody (60B1220.1)
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL | View 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:500 | - |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:500 | - |
| Immunohistochemistry - Free Floating (IHC (Free)) | 1:500 | - |
| Immunocytochemistry (ICC/IF) | 1:10 | View 2 publications |
| Flow Cytometry (Flow) | 1–5 µg/mL | - |
| Immunoprecipitation (IP) | 1:10–1:500 | View 1 publication |
| ChIP assay (ChIP) | 1:20–1:1,000 | View 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Sheep, Pig, Rat |
| Published Species | Gerbil, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 60B1220.1 |
| Immunogen | Synthetic peptide corresponding to amino acids 637–650 (EKDDREDKENAFKR) of human Dnmt1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C and avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2537688 |
Product Specific Information
Suggested positive control: human kidney (IHC), mouse ES cells (WB).
Target Information
This gene encodes a subunit of the augmin complex, which regulates centrosome and mitotic spindle integrity and is necessary for the completion of cytokinesis. The encoded protein interacts with ubiquitin C-terminal hydrolase 37. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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