Thermo Fisher Scientific CD8 Monoclonal Antibody (15-11C5)

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

1:50

-

Flow Cytometry (Flow)

1:50

-

Product Specifications

Species Reactivity

Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

15-11C5

Immunogen

15-11C5 is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from Balb/c mice immunized with WAG/Rij spleen cells. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD8,uniProtId:P01731-1,ncbiNodeId:10090,antigenRange:1-247,antigenLength:247,antigenImageFileName:MA5-49022_CD8_P01731-1_House_mouse.svg,antigenImageFileNamePDP:MA5-49022_CD8_P01731-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

100 µg/mL

Purification

Protein G

Storage buffer

PBS with 50% glycerol

Contains

0.01% sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_3093501

Product Specific Information

The monoclonal antibody recognizes rat CD8 antigen (MW 34 kDa and 39 kDa) and is reactive with all common inbred strains. 15-11C5 is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from Balb/c mice immunized with WAG/Rij spleen cells.

Target Information

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.