Thermo Fisher Scientific CD15 Chimeric Recombinant Rabbit Monoclonal Antibody (BRA-4F1)
CD15 항원을 인식하는 재조합 토끼 단클론 항체로 인간 시료에 반응함. IHC, ICC/IF, Flow Cytometry, IP 등에 사용 가능. 고순도 Protein A 정제 및 HEK293 발현 시스템 기반. 장기 보관 시 -20°C에서 안정적으로 유지.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | 10 µg/mL | - |
| Flow Cytometry (Flow) | Assay-dependent | - |
| Immunoprecipitation (IP) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BRA-4F1 |
| Immunogen | This antibody was raised by immunizing mice with human CD15. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3074708 |
Product Specific Information
Specificity: This antibody is specific for CD15. It also shows cross-reactivity with the CD15 antigen from other species.
Target Information
CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisaccharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins, and proteoglycans of various cell types such as granulocytes, mast cells, monocytes, macrophages, gastric mucosa cells, nervous system, and various tumor cells.
Variants include sialyl-Lewis x and sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation via calcium-dependent Le(x)-Le(x) interactions, involved in processes like embryo compaction and teratocarcinoma cell autoaggregation.
Sialyl-Le(x) and sialyl-Le(a) serve as ligands for selectins. CD15 expression is widely used to confirm Hodgkin’s disease diagnosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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