Thermo Fisher Scientific SPHK1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

2-3 µg/mL

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide sequence (DVDLESEKYRRLGE) corresponding to the internal amino acids of SPHK1 (aa 176-190). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SPHK1,uniProtId:Q9NYA1-1,ncbiNodeId:9606,antigenRange:176-190,antigenLength:384,antigenImageFileName:PA5-18905_SPHK1_Q9NYA1-1_Goat.svg,antigenImageFileNamePDP:PA5-18905_SPHK1_Q9NYA1-1_Goat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_10981201

Product Specific Information

This antibody is predicted to react with bovine, canine, mouse and rat based on sequence homology.

This antibody is tested in Peptide ELISA: antibody detection limit dilution 16,000.

Target Information

SPHK1, along with SPHK2, catalyzes the phosphorylation of sphingosine producing the signaling molecule sphingosine-1-phosphate (S1P). S1P signals through G-protein coupled receptors and controls diverse cellular processes. Inhibitors of SPHKs are viewed as potential therapeutics for the treatment of cancer. SPHK1 catalyzes the phosphorylation of sphingosine to sphingosine 1 phosphate (S1P), an important signaling molecule with intra- and extracellular functions. Inside the cell S1P acts as a signaling molecule like other sphingolipid metabolites like ceramide and sphingosine. S1P has been implicated in regulating cell differentiation, calcium mobilization from intracellular stores, and apoptosis. The cell surface receptors for S1P are the EDG family of G protein-coupled receptors (S1P Receptors).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.