Thermo Fisher Scientific H3K23me2 Polyclonal Antibody
H3K23me2 다이메틸화 히스톤 H3 인식 폴리클로날 항체로, WB, IHC, ELISA, IP에 적합합니다. Rabbit IgG 유래로 친화 크로마토그래피 정제되었으며, -20°C 보관 시 안정적입니다. 인간, 마우스, 랫, C. elegans에 반응합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunohistochemistry (IHC) | 1:100 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | C. elegans, Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Histone H3 [Dimethyl Lys23] affinity purified antibody, produced by repeated immunizations with a synthetic peptide surrounding the K23me2 site of human Histone H3. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate (pH 7.2), 30% glycerol, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Product is stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
A BLAST analysis suggests cross-reactivity with Human, Mouse, Rat, and C. elegans based on 100% sequence homology. Cross-reactivity with Histone H3 K23me2 from other sources has not been determined.
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the nucleosome structure. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Post-translational modifications of histones can alter chromatin structure or facilitate the binding of specific transcription factors. The N-terminal tail of histone H3 can undergo methylation, acetylation, or phosphorylation, influencing various cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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