Thermo Fisher Scientific APE1 Monoclonal Antibody (12H1)
APE1 단백질을 인식하는 마우스 단일클론 항체로, Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합함. 고순도 항원 친화 크로마토그래피로 정제되었으며, 인간 및 마우스 반응성. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 12H1 |
| Immunogen | Recombinant protein within Human APE1 aa 20–350 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 0.2% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931511 |
Target Information
Mammalian apurinic/apyrimidinic endonuclease (APE/ref-1) is a multifunctional, bipartite enzyme crucial for DNA repair and redox regulation of transcription factors such as Fos, Jun, NF-κB, Myb, HIF-1α, CREB, and Pax.
APE is involved in repairing abasic sites caused by spontaneous loss, oxidative damage, chemotherapy, ionizing radiation, UV irradiation, and DNA glycosylase activity.
It shows differential expression during development and across tissues, with diverse subcellular localization supporting interaction with various cellular proteins beyond nuclear DNA repair.
Regulation occurs via phosphorylation by casein kinase II, and expression increases upon reoxygenation of hypoxic cells or in malignant tissues, while decreased expression correlates with apoptosis induction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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