Thermo Fisher Scientific COX2 Monoclonal Antibody (A3F7)
COX2 단백질을 인식하는 마우스 단클론 항체로, Western blot, IHC, Flow cytometry에 적합합니다. Human 및 Rat 시료에 반응하며, Protein G 정제 및 액상 형태로 제공됩니다. 염증 및 암 관련 연구에 유용한 연구용 항체입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | A3F7 |
| Immunogen | Synthetic peptide within C-terminal of human COX2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 0.2% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931506 |
Target Information
COX2 converts arachidonate to prostaglandin H2 (PGH2), a key step in prostanoid synthesis including inflammatory prostaglandins. The conversion involves two reactions:
- Cyclooxygenase (COX) reaction – converts arachidonate to prostaglandin G2 (PGG2)
- Peroxidase reaction – reduces PGG2 to prostaglandin H2 (PGH2)
COX2 is constitutively expressed in tissues such as endothelium, kidney, and brain, and is up-regulated under pathological conditions like cancer and inflammation. Its up-regulation is associated with increased cell adhesion, phenotypic changes, resistance to apoptosis, and tumor angiogenesis.
In cancer cells, COX2 is crucial for prostaglandin E2 (PGE2) production, influencing motility, proliferation, and apoptosis resistance. COX2 activity is naturally inhibited by calcitriol (active Vitamin D). Glucocorticoids repress PTGS2 gene activity by interfering with transcription initiation and elongation. COX2 is a known target of NSAIDs such as aspirin, which reduce inflammation-driven pain and swelling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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