Thermo Fisher Scientific IKK-alpha Polyclonal Antibody
Rabbit polyclonal antibody targeting IKK-alpha (CHUK), validated for WB, IHC, and IP applications. Supplied as an unconjugated liquid form at 200 µg/mL concentration. Recommended storage at 4°C; shelf life 1 year. Suitable for inflammation and cell sur...
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Applications
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000 | |
| Immunohistochemistry (IHC) | 1:20–1:100 | Epitope retrieval with citrate buffer pH 6.0 recommended for FFPE tissue sections |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Between amino acids 350–400 of human IKK alpha (UniProt ID: O15111-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, use citrate buffer (pH 6.0) for epitope retrieval on FFPE tissue sections
Target Information
CHUK (IKK alpha) is a serine/threonine kinase involved in signal transduction and cellular communication. It forms part of the cytokine-activated protein complex that regulates the NF-kappa B pathway. Together with IKK beta and NEMO (IKK gamma), it phosphorylates IkB proteins, leading to their ubiquitin-mediated degradation. IKK alpha plays a crucial role in inflammation and cell survival processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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