Thermo Fisher Scientific LC3B Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-2 µg/mL

View 23 publications 23 publications

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:400

-

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:200

View 2 publications 2 publications

Flow Cytometry (Flow)

Assay-Dependent

-

ELISA (ELISA)

Assay-Dependent

-

Immunoprecipitation (IP)

20 µg/500 µg of protein

View 1 publication 1 publication

ChIP assay (ChIP)

Assay-Dependent

-

in situ PLA (PLA)

Assay-Dependent

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Zebrafish, Avian, Bacteria, Bovine, Dog, Chicken, Guinea pig, Hamster, Human, Invertebrate, Mouse, Non-human primate, Pig, Rabbit, Rat

Published species

Human, Mouse, Non-human primate, Rat, Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide between amino acids 1-100 of the human LC3 protein. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:LC3B,uniProtId:A6NCE7-1,ncbiNodeId:9606,antigenRange:1-100,antigenLength:125,antigenImageFileName:PA1-16930_LC3B_A6NCE7-1_Rabbit.svg,antigenImageFileNamePDP:PA1-16930_LC3B_A6NCE7-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2281384

Product Specific Information

The reaction with mouse LC3 is weaker than human.

The immunogen for this antibody production has 100% sequence homology with rat and zebrafish, 92% with mouse, and 91% with bovine proteins.

Target Information

LC3B (Autophagy Marker Light Chain 3B, MAP1A/MAP1B LC3 B) in humans, is encoded by the gene MAP1LC3B (Microtubule-associated proteins 1A/1B light chain 3B). LC3B is associated with microtubule assembly and important is in neurogenesis. Recent studies indicate that LC3B plays a vital role in autophagy, a process that involves the bulk degradation of cytoplasmic component. Three human LC3 isoforms undergo post-translational modifications during autophagy. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). LC3B is a microtubule-associated protein that mediate the physical interactions between microtubules and components of the cytoskeleton. LC3B may play a role in processes involving cancer, aging, metabolic and neurodegenerative disorders and cardiovascular/pulmonary diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.