Thermo Fisher Scientific LC3B Polyclonal Antibody
LC3B 단백질을 표적으로 하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, Western blot, IHC, ICC 등 다양한 응용에 적합. 인간, 생쥐, 쥐 등 여러 종에 반응하며 자가포식 연구에 유용. 항원 친화 크로마토그래피로 정제된 고순도 액상 항체.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5–2 µg/mL | View 23 publications |
| Immunohistochemistry (IHC) | – | View 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:400 | – |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:200 | View 2 publications |
| Flow Cytometry (Flow) | Assay-Dependent | – |
| ELISA | Assay-Dependent | – |
| Immunoprecipitation (IP) | 20 µg/500 µg of protein | View 1 publication |
| ChIP assay (ChIP) | Assay-Dependent | – |
| in situ PLA (PLA) | Assay-Dependent | – |
| Miscellaneous PubMed (Misc) | – | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Zebrafish, Avian, Bacteria, Bovine, Dog, Chicken, Guinea pig, Hamster, Human, Invertebrate, Mouse, Non-human primate, Pig, Rabbit, Rat |
| Published Species | Human, Mouse, Non-human primate, Rat, Zebrafish |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide between amino acids 1–100 of the human LC3 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2281384 |
Product Specific Information
- The reaction with mouse LC3 is weaker than with human LC3.
- The immunogen has 100% sequence homology with rat and zebrafish, 92% with mouse, and 91% with bovine proteins.
Target Information
LC3B (Autophagy Marker Light Chain 3B, MAP1A/MAP1B LC3B) is encoded by the MAP1LC3B gene in humans. LC3B is associated with microtubule assembly and plays an important role in neurogenesis and autophagy.
Autophagy is a process for degrading cytoplasmic components, including enzymes and organelles, especially during nutrient starvation. LC3B mediates interactions between microtubules and cytoskeletal components and may be involved in cancer, aging, metabolic, neurodegenerative, and cardiovascular/pulmonary diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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