Thermo Fisher Scientific LC3A/LC3B Polyclonal Antibody
LC3A 및 LC3B 단백질을 모두 인식하는 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF 등 다양한 응용에 사용 가능. 자가포식(autophagy) 연구에 유용하며, 고순도의 항원 친화 크로마토그래피로 정제됨. -20°C 이하에서 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 2 µg/mL | 21 publications |
| Immunohistochemistry (IHC) | - | 4 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:400 | 1 publication |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:300 | 4 publications |
| Flow Cytometry (Flow) | Assay-dependent | - |
| ELISA | Assay-dependent | - |
| Immunoprecipitation (IP) | 20 µg/500 µg lysate | - |
| ChIP assay (ChIP) | Assay-dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Fish, Human, Mouse, Plant, Rat, Zebrafish |
| Published Species | Fish, Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to a region between residues 25–121 of human LC3 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2137583 |
Product Specific Information
- This antibody detects both LC3A and LC3B.
- Western blot bands are observed at approximately 19 kDa (LC3-I) and 17 kDa (LC3-II).
- The observed molecular weight may vary due to post-translational modifications, cleavage, or charge differences.
- Recommended positive controls:
- LC3B overexpression lysate
- Mouse brain whole tissue lysate (adult, normal)
- Human brain whole tissue lysate (adult, normal)
- Neuro2a whole cell lysate
- Neuro2a and HeLa chloroquine-treated/untreated cell lysates
- Predicted to react with Bovine based on 100% sequence homology.
Target Information
Autophagy is an intracellular degradation process where cytoplasmic components, including organelles, are sequestered within double-membrane vesicles (autophagosomes) that fuse with lysosomes for degradation.
LC3 (microtubule-associated protein 1 light chain 3) is a mammalian homologue of Atg8 and a key marker of autophagy. LC3-I is cytosolic, while LC3-II is membrane-bound and enriched in autophagic vacuoles.
Detection of LC3-I to LC3-II conversion serves as a sensitive marker for monitoring autophagy in mammalian cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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