Thermo Fisher Scientific LC3A/LC3B Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 21 publications 21 publications

Immunohistochemistry (IHC)

-

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:400

View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

-

Immunocytochemistry (ICC/IF)

1:100-1:300

View 4 publications 4 publications

Flow Cytometry (Flow)

Assay-Dependent

-

ELISA (ELISA)

Assay-Dependent

-

Immunoprecipitation (IP)

20 µg/500 µg of lysate

-

ChIP assay (ChIP)

Assay-Dependent

-

Product Specifications

Species Reactivity

Dog, Fish, Human, Mouse, Plant, Rat, Zebrafish

Published species

Fish, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to a region between residues 25-121 of human LC3 protein. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:LC3A/LC3B,uniProtId:Q9GZQ8-1,ncbiNodeId:9606,antigenRange:25-121,antigenLength:125,antigenImageFileName:PA1-16931_LC3ALC3B_Q9GZQ8-1_Rabbit.svg,antigenImageFileNamePDP:PA1-16931_LC3ALC3B_Q9GZQ8-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:LC3A/LC3B,uniProtId:A6NCE7-1,ncbiNodeId:9606,antigenRange:25-121,antigenLength:125,antigenImageFileName:PA1-16931_LC3ALC3B_A6NCE7-1_Rabbit.svg,antigenImageFileNamePDP:PA1-16931_LC3ALC3B_A6NCE7-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2137583

Product Specific Information

This antibody detects both LC3A and LC3B.|Western blot bands are seen at ~19 kDa, representing LC3-I, and ~17 kDa, representing LC3-II. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Recommended positive controls: LC3B overexpression lysate, mouse brain whole tissue lysate (adult whole normal), human brain whole tissue lysate (adult whole normal), Neuro2a whole cell lysate, Neuro2a Chloroquine-treated/untreated cell lysate, HeLa chloroquine-treated/untreated cell lysate

This antibody is predicted to react with Bovine based on 100% sequence homology.

Target Information

Autophagy is a process of intracellular bulk degradation in which cytoplasmic components, including organelles, are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. During macroautophagy, the sequestering vesicles, termed autophagosomes, fuse with the lysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) into the lumen of the degradative compartment. There are 16 proteins participating in the autophagy pathway in human. The autophagy protein LC3, a mammalian homologue of Atg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. LC3 is the only known mammalian protein identified that stably associates with the autophagosome membranes. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. The detection of LC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagy in mammalian cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.