Thermo Fisher Scientific CD90.2 (Thy-1.2) Monoclonal Antibody (30-H12), Functional Grade, eBioscience

Thermo Fisher Scientific CD90.2 (Thy-1.2) Monoclonal Antibody (30-H12), Functional Grade

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• APC-eFluor 780
• Biotin
• FITC
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 780
• Request custom conjugation available

Product Specific Information

The 30-H12 monoclonal antibody reacts with mouse CD90.2 (Thy-1.2), a GPI-linked membrane molecule expressed by mouse thymocytes, mature T cells, and neurons in CD90.2-expressing strains (BALB/c, CBA, C3H, C57BL/6, C58/J, SJL, etc.). Cells from CD90.1-expressing strains (PL, AKR) do not stain with 30-H12.
CD90 is involved in T cell adhesion and signal transduction regulation.

Applications Reported:

• Flow cytometric analysis
• In vivo and in vitro depletion

Applications Tested:

• Tested by flow cytometric analysis of mouse splenocytes
• Recommended ≤ 0.125 µg/test (100 µL final volume)
• Cell number range: 10⁵–10⁸ cells/test
• Antibody titration recommended for optimal performance

Storage and Handling:

• Use in sterile environment
• 0.2 µm post-manufacturing filtration

Purity and Quality:
| Parameter | Specification |
|------------|----------------|
| Purity | >90% (SDS-PAGE) |
| Endotoxin Level | <0.001 ng/µg antibody (LAL assay) |
| Aggregation | <10% (HPLC) |

Target Information

CD90.2 (Thy-1) is a GPI-linked glycoprotein of the Immunoglobulin superfamily. It is expressed in murine T cells, thymocytes, neural cells, Kupffer’s cells, and fibroblasts. Thy-1 may play roles in cell-cell or cell-ligand interactions during synaptogenesis and other brain events.
CD90.2 is an 18 kDa plasma membrane antigen expressed by hematopoietic cells and neurons, highly expressed in connective tissue, fibroblast and stromal cell lines, and tumor endothelial cell lines. In mice, CD90.2 is expressed on all thymocytes and peripheral T lymphocytes.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.