Thermo Fisher Scientific RTCA Polyclonal Antibody

Applications

• Western Blot (WB): 0.2–1 µg/mL

Product Specifications

Product Specific Information

• Immunogen sequence:
  VQKIRAGRST PGLRPQHLSG LEMIRDLCDG QLEGAEIGST EITFTPEKIK

• Storage guidance:
  For short-term use, store at 2–8°C up to 1 week.
  For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

• Predicted homology:
  Cow: 100%
  Dog: 100%
  Guinea Pig: 100%
  Horse: 100%
  Human: 100%
  Mouse: 100%
  Rabbit: 100%
  Rat: 100%
  Zebrafish: 100%

Target Information

RTCD1 catalyzes the conversion of 3′-phosphate to a 2′,3′-cyclic phosphodiester at the end of RNA.
The enzyme acts through three steps:

1. Adenylation of the enzyme by ATP
2. The enzyme acts on RNA-N3′P to produce RNA-N3′PP5′A
3. A non-catalytic nucleophilic attack by the adjacent 2′ hydroxyl on the phosphorus in the diester linkage to produce the cyclic end product

The biological role of this enzyme is not fully understood but is likely associated with RNA processing. Two isoforms are known.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.