Thermo Fisher Scientific IL-23 p19 Monoclonal Antibody (fc23cpg), eFluor 660, eBioscience

Thermo Fisher Scientific IL-23 p19 Monoclonal Antibody (fc23cpg), eFluor 660, eBioscience

Applications

• Intracellular staining
• Flow cytometric analysis

Tested Dilution: ≤ 0.06 µg/test
Publications: View 9 publications

Product Specifications

Product Specific Information

This fc23cpg monoclonal antibody reacts with the p19 subunit of mouse IL-23, a heterodimeric cytokine composed of p40 and p19 subunits. IL-23 signals through a heterodimeric receptor expressed on activated T cells, macrophages, and dendritic cells, sharing IL-12Rβ1 with IL-12 but possessing a unique IL-23R chain. Binding of IL-23 activates Jak/STAT pathways similar to IL-12, but induces distinct immune responses such as RORγt expression and Th17 differentiation.

Dendritic cells and macrophages produce IL-23 in response to TLR2, TLR4, TLR8 ligands, and Dectin-1 agonists. Expression levels vary depending on donor and stimulation conditions. Recent studies indicate that the p19 subunit may exist independently of p40 association.

Applications Reported: Intracellular staining and flow cytometric analysis
Applications Tested: Flow cytometry, ≤ 0.06 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)

eFluor® 660 replaces Alexa Fluor® 647, emits at 659 nm, and is excited by a red laser (633 nm).
Excitation: 633–647 nm Emission: 668 nm Laser: Red
Filtration: 0.2 µm post-manufacturing filtered

Target Information

IL-23 is a heterodimeric cytokine consisting of the IL-12 p40 subunit linked to p19. The p19 subunit alone is biologically inactive. IL-23 interacts with IL-12Rβ1 and a unique IL-23R subunit containing a STAT4-binding domain. It is secreted by activated dendritic cells and exhibits distinct biological activities compared to IL-12.

In mice, IL-23 induces proliferation of memory T cells and production of IL-17, but not IFN-γ. In humans, IL-23 promotes memory T cell proliferation and moderate IFN-γ production, showing partially overlapping functions with IL-12.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Additional Formats

• Alexa Fluor 488
• PerCP-eFluor 710
• Request custom conjugation

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