Thermo Fisher Scientific PP2A alpha Monoclonal Antibody (F.451.5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:25

-

Immunocytochemistry (ICC/IF)

1:100

-

Immunoprecipitation (IP)

1:25

-

Product Specifications

Species Reactivity

Fruit fly, Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

F.451.5

Immunogen

Synthetic peptide corresponding to the sequence of human PP2A C subunit protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

16 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10984911

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with other PP2A subunits.

Target Information

In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunits have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C. The PP2A family comprises subfamily members PP2A alpha and PP2A beta. An additional protein phosphatase catalytic subunit, PPX (also known as PP4) is a putative member of a novel PP family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.