Thermo Fisher Scientific TIMP4 Recombinant Rabbit Monoclonal Antibody (13H4L13)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

-

Immunocytochemistry (ICC/IF)

1 µg/mL

-

Flow Cytometry (Flow)

2-4 µg/1x10^6 cells

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

13H4L13

Immunogen

proprietary

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532470

Product Specific Information

This antibody is predicted to react with bat, bovine, canine, equine, non-human primate, mouse, rabbit and rat based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Tissue inhibitor of metalloproteinase 4 (TIMP4) is encoded by the gene TIMP4 located on chromosome 3. It belongs to the family of TIMP genes and is known to inhibit the activity of matrix metalloproteinases. Matrix metalloproteinases (MMP) are a group of proteinases that degrade the components of extracellular matrix. TIMP4 irreversibly binds to MMPs and inactivates them with the help of zinc as a cofactor. The netrin domain of TIMP4 is involved in platelet recruitment and aggregation. Other biological functions include response to cytokine and peptide hormone stimuli and involvement with the ovulation cycle and CNS development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.