Ion Torrent™

Oncomine™ IGH FR1-J Assay, DNA

The Oncomine IGH FR1-J Assay is a targeted next-generation sequencing (NGS) assay for detection of somatic hypermutation (SHM) in the자세히 알아보기

The Oncomine IGH FR1-J Assay is a targeted next-generation sequencing (NGS) assay for detection of somatic hypermutation (SHM) in the variable region of the immunoglobulin heavy chain of B-cell receptors. SHM increases the affinity of the B-cell receptor for antigen, post-VDJ recombination, and is frequently a region of interest in CLL research. Primers have been specifically designed to target framework 1 and joining gene regions of the immunoglobulin heavy chain, resulting in an amplicon ∼300 bp in length. When combined with Ion Reporter Software, you can easily analyze your data and create reports within a short period of time.

Key features of the Oncomine IGH FR1-J Assay:
• Sequencing of FR1-J region of IGH chain
• Detects SHM in CLL samples with high degree of sensitivity
• Optimized Oncomine informatics for easy, all-inclusive analysis and publication-ready data plots

The kit provides reagents for library construction, including dNTPs. Ion AmpliSeq libraries can be generated from DNA extracted from fresh/frozen tissue, bone marrow, whole blood, peripheral blood leukocytes, PBMCs, and sorted B cells with as little as 50 ng of nucleic acid per pool. The assay is optimized for sequencing on the Ion GeneStudio S5 System with the Ion 530 Chip to enable up to eight samples per run.

사양

제어 템플릿dNTP mix

제어 유형Ion AmpliSeq Library Plus Kit

샘플 종류DNA

Sequencing TypeGenome and DNA Sequencing

용도(장비)GeneStudio

용도(애플리케이션)Sequencing

라이브러리targeted sequencing

반응 수24

수량24 reactions

배송 조건Dry Ice

Unit SizeEach