Thermo Fisher Scientific Oncomine IGH FR1-J Assay, DNA

Thermo Fisher Scientific Oncomine™ IGH FR1-J Assay, DNA

The Oncomine IGH FR1-J Assay is a targeted next-generation sequencing (NGS) assay designed for detection of somatic hypermutation (SHM) in the variable region of the immunoglobulin heavy chain of B-cell receptors.
SHM increases the affinity of the B-cell receptor for antigens after VDJ recombination and is frequently a region of interest in chronic lymphocytic leukemia (CLL) research.

Primers in this assay are specifically designed to target framework 1 (FR1) and joining gene (J) regions of the immunoglobulin heavy chain, producing an amplicon of approximately 300 bp.
When combined with Ion Reporter™ Software, users can easily analyze sequencing data and generate comprehensive reports quickly.

Key Features

• Sequencing of FR1-J region of IGH chain
• Detects SHM in CLL samples with high sensitivity
• Optimized Oncomine informatics for easy, all-inclusive analysis
• Publication-ready data visualization and reporting
• Compatible with Ion GeneStudio™ S5 System and Ion 530 Chip (up to 8 samples per run)

Application and Sample Compatibility

The kit provides reagents for library construction, including dNTPs.
Ion AmpliSeq™ libraries can be generated from DNA extracted from:

• Fresh or frozen tissue
• Bone marrow
• Whole blood
• Peripheral blood leukocytes
• PBMCs
• Sorted B cells

Requires as little as 50 ng of nucleic acid per pool.

Specifications

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