Thermo Fisher Scientific CD184 (CXCR4) Monoclonal Antibody (2B11), Brilliant Ultra Violet 805, eBioscience

Applications

• Tested Application: Flow Cytometry
• Tested Dilution: 1.0 µg/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• Functional Grade
• PE
• PE-Cyanine5.5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 600
• Request custom conjugation

Product Specific Information

The 2B11 monoclonal antibody reacts with mouse and human CXCR4 (CD184, Fusin, LESTR, HUMSTR). CXCR4 is a G-protein-coupled chemokine receptor functioning as a co-receptor for X4 HIV-1 entry into CD4+ cells and plays a role in lymphocyte migration. Its ligand is SDF1. CXCR4 is essential for granulocyte progenitor maintenance and B cell development, with differing expression patterns between mouse and human tissues.

The 2B11 clone recognizes the amino terminus of CXCR4 and induces receptor internalization without blocking SDF1a binding.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Mouse thymocytes, ≤1.0 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)

Brilliant Ultra Violet™ 805 (BUV805) emits at 797 nm and is suitable for cytometers equipped with a 355 nm UV laser. Ensure instrument compatibility.

Recommended Buffers

When using multiple polymer dye-conjugated antibodies, use:

• Super Bright Complete Staining Buffer (Product # SB-4401)
• Brilliant Stain Buffer (Product # 00-4409-75)

Handling Notes

• Light Sensitivity: Protect from light to prevent photo-induced oxidation.
• Fixation: Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer or 1-step Fix/Lyse Solution.
• Methanol Compatibility: Short-term methanol fixation is acceptable; avoid long-term methanol exposure (>1 hour).
• Excitation/Emission: 355 nm / 797 nm (UV Laser).

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CXCR4 (Fusin, LESTR, HUMSTR) is a seven-transmembrane G-protein-coupled receptor acting as a co-receptor for HIV-1 and HIV-2 entry into CD4+ cells. It binds CXCL12/SDF-1, mediating calcium signaling and MAPK activation, and plays roles in hematopoiesis, cardiac development, vascularization, and CNS neuron survival. CXCR4 also mediates LPS-induced inflammatory responses. Antibodies against CXCR4 can block HIV-1 and HIV-2 fusion and infection.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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