Thermo Fisher Scientific BAG3 Polyclonal Antibody
Human BAG3 단백질을 인식하는 Goat Polyclonal Antibody로 Western blot 및 IHC(Paraffin) 분석에 적합. 합성 펩타이드 면역원 기반으로 제작되었으며, 액상 형태로 0.5 mg/mL 농도. 연구용으로만 사용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.3–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 7 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide sequence (SSMTDTPGNPAAP) corresponding to the C-terminus amino acids of BAG3 (aa 563–575) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_10982877 |
Product Specific Information
This antibody is tested in Peptide ELISA with an antibody detection limit dilution of 1:2000.
Target Information
BAG3 belongs to the BAG protein family that competes with Hip for binding to the Hsc70/Hsp70 ATPase domain and promotes substrate release. All BAG proteins possess an approximately 45-amino acid BAG domain near the C-terminus but differ in their N-terminal regions.
The BAG3 protein contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. These proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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