Thermo Fisher Scientific CD144 (VE-cadherin) Monoclonal Antibody (eBioBV13 (BV13)), eFluor 660, eBioscience
VE-Cadherin(CD144)을 인식하는 eBioBV13 단클론 항체로, eFluor 660 형광 표지. Flow cytometry에 적합하며, 인간과 마우스 시료에서 검증됨. 4°C 보관, 동결 금지. 연구용으로 endothelial cell 분석 및 접착 관련 연구에 활용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 3 |
| Immunohistochemistry (IHC) | - | 5 |
| Immunocytochemistry (ICC/IF) | - | 8 |
| Flow Cytometry (Flow) | 1 µg/test | 14 |
| ChIP assay (ChIP) | - | 1 |
| Peptide Array (Array) | - | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Published species | Human, Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), eFluor™ 660, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioBV13 (BV13) |
| Conjugate | eFluor™ 660 |
| Excitation/Emission Max | 651 / 668 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Wet ice |
| RRID | AB_11219470 |
Additional Formats
- Unconjugated
- Alexa Fluor 488
- APC
- Biotin
- eFluor 450
- Functional Grade
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 600
- Custom conjugation available
Product Specific Information
Description:
The eBioBV13 (BV13) monoclonal antibody reacts with mouse VE-Cadherin (CD144). VE-Cadherin is a 120 kDa member of the type II Cadherin family with five extracellular cadherin domains. It mediates homophilic adhesion between endothelial cells and is localized at adherens junctions. VE-Cadherin is expressed throughout the vasculature and is essential for endothelial cell migration, survival, and assembly into tubular structures. VE-Cadherin⁺CD45⁻ cells may also give rise to hematopoietic cells.
Applications Reported:
Suitable for flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of bEnd.3 cells. Recommended ≤1 µg/test (in 100 µL final volume, 10⁵–10⁸ cells/test). Titrate for optimal performance.
Fluorophore Information:
eFluor® 660 replaces Alexa Fluor® 647.
- Excitation: 633–647 nm
- Emission: 668 nm
- Laser: Red
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
VE-cadherin is a calcium-dependent cell-cell adhesion glycoprotein in the cadherin superfamily, located on chromosome 16. It contains five extracellular cadherin repeats, a transmembrane region, and a conserved cytoplasmic tail. It functions in homophilic adhesion and plays a critical role in endothelial cell junction organization. An alternative splice variant has been identified, though the full-length sequence is not fully determined.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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