Thermo Fisher Scientific ATP6AP1 Polyclonal Antibody
ATP6AP1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal 항체로, WB, IHC, ICC/IF에 사용 가능. 인간, 마우스, 랫트 반응성. Affinity chromatography로 정제된 액상 형태이며, PBS/glycerol buffer에 보관. 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human ATP6AP1 (Accession Q15904), corresponding to amino acid residues L91–V141 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2851793 |
Product Specific Information
This antibody detects endogenous levels of total ATP6AP1.
Target Information
Vacuolar-type H⁺-ATPase (V-ATPase) is a multisubunit enzyme responsible for acidification of eukaryotic intracellular organelles. It pumps protons against an electrochemical gradient to synthesize ATP. The enzyme consists of a peripheral V1 domain (ATP hydrolysis) and an integral V0 domain (proton translocation).
ATP6AP1 (ATPase, H⁺ transporting, lysosomal accessory protein 1), also known as 16A, CF2, Ac45, XAP3, ATP6S1, VATPS1, or ATP6IP1, is a type I transmembrane V-ATPase accessory protein predominantly expressed in endocrine and neuronal cells. It targets the V-ATPase enzyme to specialized vacuolar systems and interacts with V0 subunits via its cytoplasmic tail. Disruption of this interaction in osteoclasts impairs bone resorption, indicating its essential role in osteoclastic function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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