Thermo Fisher Scientific NK1.1 Monoclonal Antibody (PK136), PE, eBioscience
마우스 NK1.1 항원을 인식하는 PK136 단클론 항체로, PE 형광체가 결합되어 있습니다. Flow cytometry에 최적화되어 있으며, NK 세포 및 일부 T 세포 분석에 사용됩니다. 0.25 µg/test 권장, 4°C 암소 보관.
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카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific NK1.1 Monoclonal Antibody (PK136), PE, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | 0.25 µg/test | View 137 publications |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PK136 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, Do Not Freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466049 |
Available Formats
- Unconjugated
- Alexa Fluor 561
- Alexa Fluor 660
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 496 / 615 / 661 / 805
- Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
- eFluor 450
- FITC
- Functional Grade
- PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436 / 600 / 645 / 702 / 780
- Request custom conjugation
Product Specific Information
Description:
The PK136 monoclonal antibody reacts with mouse NK1.1, an antigen expressed by natural killer (NK) cells and a subset of T cells in NK1.1-positive mouse strains (e.g., C57BL, NZB). Common strains such as BALB/c, SJL, AKR, CBA, C3H, and A do not express NK1.1. For these strains, use the DX5 monoclonal antibody (Product #14-5971).
Simultaneous staining of C57BL/6 spleen cells with PK136 and DX5 reveals coexpression of both markers by most cells, with minor DX5⁺PK136⁻ and DX5⁻PK136⁺ subsets.
Applications Reported:
Flow cytometric analysis.
Applications Tested:
Tested by flow cytometry using C57BL/6 mouse splenocytes. Recommended ≤0.25 µg/test (100 µL final volume). Cell count range: 10⁵–10⁸ cells/test. Optimize antibody titration for best performance.
Excitation / Emission:
488–561 nm / 578 nm
Compatible Lasers: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered
Target Information
NK1.1 (KLRB1, NKR-P1A, CD161):
A receptor expressed on NK cells and some T cells, belonging to the C-type lectin superfamily. It contains extracellular lectin-like motifs, a transmembrane domain, and a cytoplasmic domain, classifying it as a type II membrane protein. The receptor recognizes LLT1 (Lectin Like Transcript-1) as its functional ligand and plays a role in NK cell activation and regulation.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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