Thermo Fisher Scientific 7-Ketocholesterol Monoclonal Antibody (7E1)
7-Ketocholesterol 단백질을 특이적으로 인식하는 Mouse IgG2b 단일클론 항체. Western blot, ICC/IF, ELISA에 사용 가능. KLH에 결합된 합성 7-Ketocholesterol을 면역원으로 제작. 단백질 G 정제, PBS/glycerol buffer로 보관. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| ELISA | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7E1 |
| Immunogen | Synthetic 7-Ketocholesterol conjugated to Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2735097 |
Additional Formats
- APC (MA5-45793)
- FITC (MA5-45794)
- PE (MA5-45796)
- PerCP (MA5-45795)
- Request custom conjugation
Product Specific Information
A 1:1,000 dilution of MA5-27571 was sufficient for detection of 7-Ketocholesterol in 2 µg of 7-ketocholesterol conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Specific for 7-ketocholesterol modified proteins.
Target Information
7-Ketocholesterol is a major oxidation product of cholesterol found in human atherosclerotic plaque and is more atherogenic than cholesterol in some animal studies.
It can inhibit cholesterol 7alpha-hydroxylase, the rate-limiting step in bile acid biosynthesis, and strongly inhibit HMG-CoA reductase, the rate-limiting enzyme in cholesterol biosynthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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