Thermo Fisher Scientific Neutrophil elastase Recombinant Rabbit Monoclonal Antibody (8X4E6)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

8X4E6

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 167-267 of human Neutrophil Elastase (ELANE) (P08246). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Neutrophil elastase,uniProtId:P08246-1,ncbiNodeId:9606,antigenRange:167-267,antigenLength:267,antigenImageFileName:MA5-42901_Neutrophil_elastase_P08246-1_Rabbit.svg,antigenImageFileNamePDP:MA5-42901_Neutrophil_elastase_P08246-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2912042

Product Specific Information

Positive test controls include: THP-1, U-937.

Immunogen sequence: SVLQELNVTV VTSLCRRSNV CTLVRGRQAG VCFGDSGSPL VCNGLIHGIA SFVRGGCASG LYPDAFAPVA QFVNWIDSII QRSEDNPCPH PRDPDPASRT H

Target Information

The neutrophil form of elastase is 218 amino acids long, with two asparagine-linked carbohydrate chains (see glycosylation). It is present in azurophil granules in the neutrophil cytoplasm. There appear to be two forms of neutrophil elastase, termed IIa and IIb.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.