Thermo Fisher Scientific TDG Monoclonal Antibody (GT1511)

Applications

Western Blot (WB)

• Tested Dilution: 1:5,000–1:20,000
• Publications: -

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:100–1:1,000
• Publications: -

Product Specifications

Product Specific Information

• Keep as concentrated solution.
• Predicted reactivity: Human (99%), Mouse (88%), Rat (87%), Bovine (91%).
• Positive Control: human TDG-transfected 293T cells.
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family.
Thymine-DNA glycosylase removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine.
With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine.
This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine.
This gene may have a pseudogene in the p arm of chromosome 12.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.