Thermo Fisher Scientific 6 His Polyclonal Antibody, HRP
6x His 태그 단백질 검출용 HRP 결합 폴리클로날 항체. Western blot, ICC/IF, ELISA에 최적화. 항원 친화 크로마토그래피로 정제. 높은 민감도와 특이성 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:30,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:500 |
| ELISA | 1:10,000–1:100,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | 6-His (HHHHHH) conjugated to KLH |
| Conjugate | HRP |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.1% Pro-Clean 400 |
| Storage Conditions | 2–8°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH). It is commonly expressed at the N- or C-terminal regions of recombinant proteins to enable isolation or purification by immobilized metal affinity chromatography (IMAC).
Epitope tagging allows detection of recombinant proteins by fusing a known epitope to them, facilitating purification or detection when no specific antibody exists. His-tagged proteins bind strongly and selectively to metal ions such as Ni²⁺, enabling purification to homogeneity using Ni²⁺-NTA adsorbents.
His tag-specific antibodies are valuable tools for detection, purification, and co-immunoprecipitation of His-tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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